منابع مشابه
Studies on Enzyme Action
The hydrolyzing actions of various preparations of the adult eel were studied on ten esters in the usual way. The results are presented in the form of curves for the relative actions and in a table for the absolute actions obtained in one complete experiment. The separation of the enzyme material in some cases into an active portion and a co-enzyme, the mixture showing greater actions on some e...
متن کاملENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
متن کاملEnzyme Regulation in Crassulacean Acid Metabolism Photosynthesis : Studies on Thioredoxin-Linked Enzymes of KalanchoE daigremontiana.
Fructose-1,6-bisphosphatase (FBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) were identified and purified from the Crassulacean acid metabolism (CAM) plant, Kalanchoë daigremontiana. FBPase and SBPase showed respective molecular weights of 180,000 and 76,000, and exhibited immunological cross-reactivity with their counterparts from chloroplasts of C(3) (spinach) and C(4) (corn) plants. Ba...
متن کاملStudies on Cell Enzyme Systems
Solutions of the enzyme luciferase, extracted from Cypridina, were subjected at pH 6.8 to temperatures from 40-55 degrees C. for times up to 24 hours. After the desired exposures samples were cooled rapidly to room temperature, mixed with luciferin, and the first order velocity constants (representing luciferase activity) of the resulting luminescent reactions were determined by a photo electri...
متن کاملStudies on the Enzyme Enolase*
Enolase catalyzes the interconversion of u-glyceric acid 2-phosphat,e and enolpyruvic acid phosphate,’ and, as a participant of Embden-Meyerhof glycolysis and fermentation, is widely distributed in living cells. The enzyme was first purified by Warburg and Christian (1)) who demonstrated its metal activation and made the initial studies of the kinetics and the thermodynamics of the enolase reac...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1975
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.19.361